Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
C154A | activity similar to wild-type | Escherichia coli |
C154A | mutant shows high levels of activity | Escherichia coli |
C154S | no activity | Escherichia coli |
C154S | complete loss of activity, mutant is capable of binding to substrate tRNAPhe | Escherichia coli |
C169A | activity similar to wild-type | Escherichia coli |
C169A | mutant shows high levels of activity | Escherichia coli |
C169S | no activity | Escherichia coli |
C169S | complete loss of activity, mutant is unable to bind substrate tRNAPhe | Escherichia coli |
C55A/C154A/C169A | activity similar to wild-type | Escherichia coli |
C55A/C154A/C169A | only a small change in both Km and Vmax parameters as compared with wild-type enzyme. These mutations cause a 1.2fold increase in Km and a 1.6fold decrease in Vmax. The overall Vmax/Km ratio is lowered by a factor of 2 for the triple mutant | Escherichia coli |
C55S | activity similar to wild-type | Escherichia coli |
C55S | maintains activity levels similar to those of wild-type enzyme | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000086 | - |
Escherichia coli tRNAPhe uridine39 | wild-type, pH 8.0, 30°C | Escherichia coli | |
0.000086 | - |
tRNA uridine39 | pH 8.0, 30°, wild-type enzyme | Escherichia coli | |
0.00011 | - |
tRNA uridine39 | pH 8.0, 30°C, mutant enzyme C55A/C154A/C169A | Escherichia coli | |
0.00011 | - |
Escherichia coli tRNAPhe uridine39 | mutant C55A/C154A/C169A, pH 8.0, 30°C | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
x * 31000, SDS-PAGE | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
tRNA uridine38-40 = tRNA pseudouridine38-40 | chemical evidence against a covalent cysteine intermediate in the rearrangement mechanism of uridine to pseudouridine | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Escherichia coli tRNAPhe uridine39 | - |
Escherichia coli | Escherichia coli tRNAPhe pseudouridine39 | - |
? | |
tRNA uridine39 | tRNAPhe with uridine at position 39 | Escherichia coli | tRNA pseudouridine39 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 31000, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
PSUI | - |
Escherichia coli |
tRNA pseudouridine synthase I | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Escherichia coli |